Succinate-resistant Mutants of Rhizobium leguminosarum
نویسندگان
چکیده
منابع مشابه
Rhizobium gone native: unexpected plasmid stability of indigenous Rhizobium leguminosarum.
Lateral transfer of bacterial plasmids is thought to play an important role in microbial evolution and population dynamics. However, this assumption is based primarily on investigations of medically or agriculturally important bacterial species. To explore the role of lateral transfer in the evolution of bacterial systems not under intensive, human-mediated selection, we examined the associatio...
متن کاملSugar Metabolism and the Symbiotic Properties of Carbohydrate Mutants of Rhizobium leguminosarum
Rhizobium leguminosarum metabolizes sugars via the Entner-Doudoroff and pentose phosphate pathways but does not have a functional Embden-Meyerhof pathway. Although some sugar catabolizing enzymes are constitutive, activities of the ‘Entner-Doudoroff enzymes vary with the carbon source. Bacteroids have complete pathways for sugar catabolism even though the specific activities of some enzymes, e....
متن کاملSymbiotic properties of C4-dicarboxylic acid transport mutants of Rhizobium leguminosarum.
The transport of succinate was studied in bacteroids of an effective, streptomycin-resistant strain (GF160) of Rhizobium leguminosarum. High levels of succinate transport occurred, and the kinetics, specificity, and sensitivity to metabolic inhibitors were similar to those previously described for free-living cells. The symbiotic properties of two transposon (Tn5)-mediated C4-dicarboxylate tran...
متن کاملCharacterization of salt-tolerant and salt-sensitive mutants of Rhizobium leguminosarum biovar viciae strain C1204b.
Spontaneous mutants of Rhizobium leguminosarum biovar viciae strain C1204b were selected for their ability to tolerate 0.2 M NaCl, a growth-inhibiting level of salt for the parental strain. Transposon-mediated salt-sensitive mutants of strain C1204b were screened for their inability to grow in 0.08 M NaCl. Quantitation of the free-amino acid pools in the mutants grown in NaCl revealed a dramati...
متن کاملRhizobium leguminosarum exoB mutants are deficient in the synthesis of UDP-glucose 4'-epimerase.
Rhizobium leguminosarum bv. viciae Exo- mutant strains RBL5523,exo7::Tn5,RBL5523,exo8::Tn5 and RBL5523,exo52::Tn5 are affected in nodulation and in the syntheses of lipopolysaccharide, capsular polysaccharide, and exocellular polysaccharide. These mutants were complemented for nodulation and for the syntheses of these polysaccharides by plasmid pMP2603. The gene in which these mutants are defec...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1981
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-126-1-237